In vitro selection of DNA aptamers against human osteosarcoma

Authors and affiliations:
Khaliunsarnai Tsogtbaatar ^1,2,*, Diana A. Sousa ², *, Debora Ferreira ², Atakan Tevlek ¹, Halil Murat Aydın ^1,3, Eda Çelik ^1,4, Ligia Rodrigues ²

¹ Institute of Science, Bioengineering Division, Hacettepe University, 06800, Ankara, Turkey

² Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal

³ Centre for Bioengineering, Hacettepe University, 06800, Ankara, Turkey

⁴ Department of Chemical Engineering, Hacettepe University, 06800, Ankara, Turkey

^* Khaliunsarnai Tsogtbaatar and Diana A. Sousa contributed equally to this work as first authors

Abstract:
Osteosarcoma is a highly malignant bone tumor, most frequently occurring in the rapid bone growth phase. Effective treatment of this disease is hindered by the lack of specific probes for early diagnosis and the fast cancer widespread. To find such probes, the cell-Systematic Evolution of Ligands by EXponential enrichment (cell-SELEX) methodology was implemented against the human osteosarcoma MG-63 cell line towards the selection of new specific aptamers. Through cell-SELEX, we isolated a DNA aptamer (OS-7.9) with high affinity against to human osteosarcoma cells, as well as to lung carcinoma and colon colorectal adenocarcinoma cell lines, which are reported as common metastasis sites of osteosarcoma. These findings suggest that OS-7.9 could recognize a common protein expressed in these cancer cells, possibly becoming a potential molecular probe for early diagnosis and targeted therapies for metastatic disease

Journal: Investigational New Drugs

Link: https://doi.org/10.1007/s10637-021-01161-y